PCR reagents

 

Name of Product

Quantity

CatalogNo

Description

GoTaq® Probe qPCR Master Mix 2 ml A6101 The master mix is provided as a ready-to-use, stabilized 2X formulation that includes all components for qPCR (except template, primers and probe). This master mix does not contain a reference dye;
10 ml A6102
GoTaq® Probe 1-Step RT-qPCR System 2 ml A6110
GoTaq® Probe 2-Step RT-qPCR System 2 ml A6120 The GoTaq® Probe 2-Step RT-qPCR System is optimized for quantitative PCR assays in the hydrolysis probe detection format.
GoTaq® qPCR Master Mix 5 ml A6001 The GoTaq® qPCR Master Mix is a ready-to-use 2X master mix for use in real-time quantitative PCR (qPCR and RT-qPCR). The system contains BRYT Green® Dye.
GoTaq® 2-Step RT-qPCR System 5 ml A6010 The GoTaq® 2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) protocol.
GoTaq® 1-Step RT-qPCR System 5 ml A6020 The GoTaq® 1-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a one-step reverse transcription-quantitative PCR (RT-qPCR) protocol in a single tube.
CXR Reference Dye 100 μl C5411
Plexor® qPCR System 200 rxns A4011 The Plexor® qPCR and qRT-PCR Systems are multiplex-capable real-time amplification systems using novel base pair chemistry.
MOPS/EDTA Buffer 3×10 ml Y5101 MOPS/EDTA Buffer is provided at pH 7.4 for resuspending and diluting primers and templates used in the Plexor® qPCR and qRT-PCR Systems.
GoScript™ Reverse Transcription System 50 rxns A5000 The GoScript™ Reverse Transcription System includes a reverse transcriptase and a specialized set of reagents for efficient synthesis of first-strand cDNA optimized for quantitative PCR amplification.
100 rxns A5001
GoScript™ Reverse Transcriptase 100 rxns A5003 GoScript™ Reverse Transcriptase is qualified for use in qPCR, including GoTaq® qPCR and Plexor® RT-qPCR systems.
500 rxns A5004
ImProm-II™ Reverse Transcription System 100 rxns A3800 The ImProm-II™ Reverse Transcription System produces efficient, robust synthesis of first-strand cDNA in preparation for PCR amplification. The components of the ImProm-II™ Reverse Transcription System can be used to reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA.
ImProm-II™ Reverse Transcriptase 10 rxns A3801
100 rxns A3802
500 rxns A3803
Reverse Transcription System 100 rxns A3500 The Reverse Transcription System provides reagents to efficiently reverse transcribe RNA into cDNA in 15 minutes. The cDNA prepared from each reaction may be used directly in multiple PCR amplifications using Taq DNA polymerase.
Magnesium Chloride Solution 1.5 ml (25 mM) A3511
Reverse Transcription 10X Buffer 1.4 ml A3561
AccessQuick™ RT-PCR System 20 rxns A1701 The AccessQuick™ RT-PCR System is an easy and convenient master mix for one-tube RT-PCR. The system increases the convenience of performing RT-PCR by combining the following components in a single tube: Tfl DNA Polymerase, dNTPs, magnesium sulfate and reaction buffer.
100 rxns A1702
500 rxns A1703
Access RT-PCR Introductory System 20 rxns A1260 The Access RT-PCR System is designed for reverse transcription (RT) and PCR amplification of a specific target RNA from total RNA or mRNA. This one-tube, two-enzyme system provides sensitive, quick and reproducible analysis of even rare RNAs.
Access RT-PCR System 100 rxns A1250
500 rxns A1280
AMV Reverse Transcriptase 300 u (10u/µl) M5101 The AMV Reverse Transcriptase(AMV RT) from Avian Myeloblastosis Virus for first-strand DNA synthesis andthermostable Tfl DNA polymerase from Thermus flavus for second-strand cDNAsynthesis and DNA amplification.
1,000 u (10u/µl) M5108
AMV Reverse Transcriptase (HC) 600 u (20–25u/µl) M9004
M-MLV Reverse Transcriptase 10,000 u (200 u/µl) M1701 Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb).
50,000 u (200 u/µl) M1705
M-MLV Reverse Transcriptase, RNase H Minus 10,000 u (100–200 u/µl) M5301 This form of M-MLV Reverse Transcriptase is genetically altered to remove the associated RNase H activity.
M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant 2,500 u M3681 Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT [H–]), Point Mutant, is an RNA-dependent DNA polymerase that can be used for cDNA synthesis with long RNA templates (>5kb).
10,000 u M3682
50,000 u M3683
Tth DNA Polymerase 100 u (5u/µl) M2101 Tth DNA Polymerase is a thermostable enzyme of approximately 94kDa isolated from Thermus thermophilus HB-8. The enzyme replicates DNA at 74°C and exhibits a half-life of 20 minutes at 95°C.
500 u (5u/µl) M2105
dATP 25 μmol (100mM) U1205 High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing and labeling. Promega dNTPs have greater than 99% triphosphate content and are provided at a concentration of 100mM in water at pH 7.5.
40 μmol (100mM) U1201
200 μmol (100mM) U1202
dGTP 25 μmol (100mM) U1215
40 μmol (100mM) U1211
200 μmol (100mM) U1212
dCTP 25 μmol (100mM) U1225
40 μmol (100mM) U1221
200 μmol (100mM) U1222
dTTP 25 μmol (100mM) U1235
40 μmol (100mM) U1231
200 μmol (100mM) U1232
Set of dATP, dCTP, dGTP, dTTP 10µmol each (100mM) U1330
25µmol each (100mM) U1420
40µmol each (100mM) U1240
200µmol each (100mM) U1410
rATP, rCTP, rGTP, rUTP, each at 10mM in separate tubes 0.5 ml (10 mM) P1221 Ribonucleotide Triphosphates (rNTPs) are provided in individual tubes and qualified for use with the Riboprobe® and HeLaScribe® Systems. The rNTPs are supplied in nuclease-free water. Purity is verified by HPLC analysis.
rATP, 10mM 0.5 ml (10 mM) P1132
rCTP, 10mM 0.5 ml (10 mM) P1142
rGTP, 10mM 0.5 ml (10 mM) P1152
rUTP, 10mM 0.5 ml (10 mM) P1162
rATP, 10mM 400 μl E6011
rUTP, 100mM 400 μl E6021
rGTP, 100mM 400 μl E6031
rCTP, 100mM 400 μl E6041
rCTP, rATP, rUTP, rGTP, 100mM each 4 × 400μl E6000
Universal RiboClone® cDNA Synthesis System 1 system C4360 The Universal RiboClone® cDNA Synthesis System contains the reagents required for synthesis of double-stranded cDNA from mRNA and subsequent ligation into a suitable vector.
Oligo(dT)15 Primer 20 μg C1101
Random Primers 20 μg C1181
Spin Columns 10 each C1281
EcoRI Adaptors 150 pmol C1291
1.2kb Kanamycin Positive Control RNA 5 μg C1381
Sephacryl® S-400 10 ml V3181
GoTaq® G2 Hot Start Polymerase 100 u (5u/µl) M7401 GoTaq® G2 Polymerase is the second generation of GoTaq® products. The enzyme comes in a variety of formats designed to provide maximum flexibility, control and convenience.
500 u (5u/µl) M7405
2500 u (5u/µl) M7406
10000 u (5u/µl) M7408
GoTaq® G2 Hot Start Green Master Mix 100 rxns M7422 The GoTaq® G2 Hot Start Master Mixes are ready-to-use mixes containing all necessary components (GoTaq® G2 Hot Start Polymerase, buffer, dNTPs and optimized magnesium)—you only need to add primer and template and go!
1000 rxns M7423
GoTaq® G2 Hot Start Colorless Master Mix 100 rxns M7432 The GoTaq® G2 Hot Start Colorless Master Mix contains no gel loading dye for use when downstream applications require fluorescence or absorbance readings without purification.
1000 rxns M7432
GoTaq® Hot Start Polymerase 100 u (5u/µl) M5001 GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity.
500 u (5u/µl) M5005
2500 u (5u/µl) M5006
10000 u (5u/µl) M5008
GoTaq® Hot Start Green Master Mix 100 rxns M5122 GoTaq® Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq® Hot Start Polymerase, magnesium, dNTPs and buffer.
1000 rxns M5123
GoTaq® Hot Start Colorless Master Mix 100 rxns M5132
1000 rxns M5133
GoTaq® Long PCR Master Mix 100 rxns M4021 GoTaq® Long PCR Master Mix contains the high-performance GoTaq® Hot Start Polymerase in a specially formulated mixture with a proprietary thermostable proofreading polymerase
GoTaqR G2 Flexi DNA Polymerase 100 u (5u/µl) M7801 The second generation of GoTaq® products, GoTaq® G2 DNA Polymerase reliably amplifies a wide range of PCR templates and provides high-performance results due to improved manufacturing processes, increased reliability and consistency. The product is available in many formats to give you maximum flexibility, control and convenience for your PCR.
500 u (5u/µl) M7805
2500 u (5u/µl) M7806
10000 u (5u/µl) M7808
GoTaq® G2 DNA Polymerase 100 u (5u/µl) M7841
500 u (5u/µl) M7845
2500 u (5u/µl) M7848
GoTaq® G2 Green Master Mix 100 rxns M7822
1000 rxns M7823
GoTaqR G2 Colorless Master Mix 100 rxns M7832
1000 rxns M7833
GoTaq® Flexi DNA Polymerase 100 u (5u/µl) M8291 Experience improved PCR performance with GoTaq® DNA Polymerase products. GoTaq® DNA Polymerase is a proprietary formulation of Taq DNA polymerase that gives robust amplification equal to and in some cases superior to that of standard Taq DNA polymerase. GoTaq® DNA Polymerase comes in a variety of formulations to give you maximum flexibility, control and convenience.
500 u (5u/µl) M8295
2500 u (5u/µl) M8296
5000 u (5u/µl) M8297
10000 u (5u/µl) M8298
GoTaq® DNA Polymerase 100 u (5u/µl) M3001
500 u (5u/µl) M3005
2500 u (5u/µl) M3008
GoTaqR Green Master Mix 100 rxns M7122
1000 rxns M7123
GoTaqR Colorless Master Mix 100 rxns M7132
1000 rxns M7133
5X Green GoTaq® Reaction Buffer 20 ml M7911 The 5X Green GoTaq® Reaction Buffer contains two dyes (a blue dye and a yellow dye) that separate during electrophoresis to show migration progress. The buffer also contains a compound that increases sample density. This means that samples can be loaded directly onto gels without the need for loading dye.
5X Colorless GoTaqR Reaction Buffer 20 ml M7921
5X Colorless GoTaqR Flexi Reaction Buffer 20 ml M8901
5X Green GoTaqR Flexi Reaction Buffer 20 ml M8911
Magnesium Chloride Solution 1.5 ml (25 mM) A3511
25 ml (25 mM) A3513
GoTaq® PCR Core System I 200 rxns M7660 The GoTaq® PCR Core Systems I and II are designed for exponential amplification of specific regions of DNA using the polymerase chain reaction. Both systems include GoTaq® DNA polymerase and PCR Nucleotide Mix, along with high-performance buffers and magnesium chloride. The GoTaq® PCR Core System II also includes the Positive Control Plasmid DNA
GoTaq® PCR Core System II 200 rxns M7665
PCR Master Mix 10 rxns M7501 PCR Master Mix is a premixed, ready-to-use solution containing Taq DNA polymerase, dNTPs, MgCl2 and reaction buffer at optimal concentrations for efficient amplification of DNA templates by PCR.
100 rxns M7502
1000 rxns M7505
Tfl DNA Polymerase 100 u (5u/µl) M1941 Tfl DNA Polymerase is a thermostable enzyme of approximately 94kDa isolated from Thermus flavus. The enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C.
1000 u (5u/µl) M1945
Pfu DNA Polymerase 100 u

(2-3u/µl)

M7741 Pfu DNA Polymerase is a thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium.
500 u

(2-3u/µl)

M7745
PCR Nucleotide Mix 200 μl (10 mM) C1141 High-quality deoxynucleotide triphosphates (dNTPs) are critical for PCR success. The PCR Nucleotide Mix is a premixed solution containing the sodium salts of dATP, dCTP, dGTP and dTTP, each at a concentration of 10mM in water at pH 7.5;
1000 μl (10 mM) C1145
dNTP Mix 200 μl (10 mM) U1511 dNTP Mix is a premixed solution containing sodium salts of dATP, dCTP, dGTP and dTTP, each at 10mM in water at pH 7.5
1000 μl (10 mM) U1515
dUTP 40 μmol (100 mM) U1191 dUTP (2´-Deoxyuridine, 5´-Triphosphate) can be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications.
Set of dATP, dCTP, dGTP, dUTP 10 μmol (100 mM) U1335
40 μmol (100 mM) U1245
pGEM®-T Vector System I 20 rxns A3600 The pGEM®-T Vector Systems are convenient systems to clone PCR products. The pGEM®-T Vector is prepared by cutting the pGEM®-5Zf(+) Vector with EcoRV and adding a 3´ terminal thymidine to both ends. The pGEM®-T Vector System II contains JM109 Competent Cells in addition to all of the pGEM®-T Vector System I components.
pGEM®-T Vector System II 20 rxns A3610
pGEM®-T Easy Vector System I 20 rxns A1360 The pGEM®-T Easy Vector Systems are convenient systems to clone PCR products. They offer all of the advantages of the pGEM®-T Vector Systems with the added convenience of recognition sites for EcoRI and NotI flanking the insertion site.
pGEM®-T Easy Vector System II 20 rxns A1380
pTARGET™ Mammalian Expression Vector System 20 rxns A1410 The pTARGET™ Mammalian Expression Vector System is a convenient system for cloning PCR products and expressing cloned PCR products in mammalian cells.
pTARGET™ Sequencing Primer 2 μg Q4461 The pTARGET™ Sequencing Primer is designed for sequencing inserts cloned into the pTARGET™ Mammalian Expression Vector (Cat.# A1410). The sequencing primer hybridizes to the region of the lacZ gene at nucleotides 1367–1344 on the pTARGET™ Vector.